香港黄大仙码报要钱料
PerkinElmer

3D细胞培养与分析

OF

3D 微组织的大量应用已经成为建立高生物相关型形态和功能模型的关键,这一模型对于明确组织形态,疾病机制研究,药物研发和组织工程都具有重大意义。

Perkinelmer 为3D 微组织应用提供了完整的解决方案,从3D微组织培养,代谢性评估试剂到先进的高内涵成像分析平台。

3D-Cell-Culture-4
3D-Cell-Culture-4

Grow 3D cell culture with speciality microplates

Choose from a range of carefully designed microplate technologies that produce uniform 3D cultures – time after time. We offer a variety of microplates and advanced surface coatings to meet the needs of unique cell types and applications.?
  • PerkinElmer CellCarrier Spheroid Ultra Low Attachment Microplates – Get consistent 3D cellular spheroid formation with minimal cell-plate adhesion, promoting single spheroid formation with robust circular formation. The optically-clear, round bottom produces high-quality imaging results.
  • Insphero GravityTRAP? ULA Plate – Ideal for long-term cultivation, the SureXchange? tapered ledge facilitates easy, medium exchange and prevents microtissue loss. The flat-bottom observation chamber enables brightfield microscopy applications and size measurements.
  • GravityPLUS? Plate - A hanging drop plate for outstanding size consistency, featuring SureDrop? microtechnology, with variation in diameter of 5% or less across an entire 96-well plate. Hanging drop spheroid formation is recommended for co-culture and primary cells.
  • Insphero GravityPLUS? Hanging-Drop Spheroid Culture System - A combination of the GravityPLUS? Plate (formulation of spheroids in a hanging drop plate), and the GravityTRAP? ULA Plate (for the capture and assay of cells).
3D InSight? Human Liver Microtissues from InSphero labeled imaged on the Operetta CLS system
3D InSight? Human Liver Microtissues from InSphero labeled imaged on the Operetta CLS system

Detect with sensitive detection and high-content imaging solutions

Our multimode microplate readers and high-content analysis systems, plus innovative reagents, provide you with highly sensitive solutions for 3D cell culture imaging and detection.
  • Opera Phenix? high-content screening system is the premier confocal solution for 3D high-content screening, enabling fast multichannel 3D acquisition at high image quality.
  • With its unique combination of technologies, the Operetta CLS? high-content analysis system provides flexibility, sensitivity, and resolution for 3D imaging.
  • With the EnSight multimode plate reader you can monitor the growth and health of your 3D cell cultures by measuring size, shape, and more using image cytometry. Combine imaging with other technologies such as AlphaLISA? to detect secreted proteins.
  • ATPlite? 3D and ATPlite 1step 3D reagent kits for cell viability and proliferation provide a simple, robust protocol for ATP-content endpoint measurements of 3D spheroids.
3D-Cell-Culture-6
3D-Cell-Culture-6

Analyze with powerful and intuitive informatics solutions to uncover new insights

Our scientific software and informatics solutions give you the tools to optimize imaging of 3D cell models and then aggregate, search, mine, and visualize critical data - and turn it into actionable insights.
  • Turn data into understanding with the intuitive Harmony? imaging and analysis software for the Opera Phenix and Operetta CLS systems. You can accelerate 3D image acquisition and analysis, better understand spatial relationships, discover new perspectives not visible in 2D and measure volume and morphology in 3D. Optional PreciScan? intelligent acquisition lets you pre-scan to locate your sample in the well for significantly reduced acquisition and analysis times.
  • Manage the large amounts of data that high-content analysis generates with Columbus? image data storage and analysis software. Add cluster computing capabilities for even greater power when acquiring large datasets for working with 3D samples.
Business Insights (6)
Filters:

1-6 的 6 Business Insights

  • 应用文献

    Phenotypic Profiling of Autophagy using the Opera Phenix High-Content Screening System

    Cells constantly sense their environment and their response is a spatio-temporal summation of all signals. To maintain physiological stability, cells need to adjust to environmental changes, a process called homeostasis. One of the most important processes involved in maintaining homeostasis is autophagy, and its significance was recognized by the award of the Nobel Prize for Physiology in 2016 to Yoshinori Ohsumi for the discovery of its underlying mechanisms. Although this is not fully understood, it is believed that autophagy can prevent tumor development by degrading, for example, damaged organelles and protein aggregates.

  • 应用文献

    用高内涵筛选系统进行FRET测定

    在这个实验中,我们建立了一种快速稳定的基于FRET的高内涵检测...

  • 应用文献

    Imaging Bile Canaliculi in 3D Liver Microtissues using the Opera Phenix HCS System

    Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated with an increased risk of cholestatic liver injury. Here, we describe a 3D high-content screening assay to study hepatobiliary transporter function in InSphero human liver microtissues. Confocal imaging and automated image analysis were used to quantify BSEP and MRP-2-mediated efflux of fluorescent substrates into bile canaliculi.

  • 应用文献

    Phenotypic Analysis of Hypertrophy in Human iPSC-Derived Cardiomyocytes

    Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. Learn how: The Opera Phenix system has been applied in the field of neurodegenerative diseases. In this assay, the Opera Phenix system is 4 times faster than the previous Opera? system. Primary neuron morphology is analyzed in a straightforward approach using Harmony software. Careful assay optimization can increase throughput, and minimize the data burden, without compromising assay performance.

  • 应用文献

    Analyzing ERK Signal Transduction in Live Cells Using a FRET-Based Biosensor

    Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ERK signaling pathway is altered in various cancer types and is frequently investigated as a target for therapeutic intervention. This application note describes how a live cell FRET assay to study ERK signaling was performed on the Operetta CLS? high-content analysis system. The optimized design of the FRET-based biosensor, the high-quality imaging of the Operetta CLS system and the easy-to-use image analysis tools of the Harmony? software contribute to the robustness of the high-content assay.

  • 案例分析

    High-Content Analysis of Drug-Induced Oligodendrocyte Differentiation Promoting Remyelination in Multiple Sclerosis

    One of the greatest challenges in multiple sclerosis (MS) therapy is the halting or reversal of the failure of remyelination in the brain in order to reverse disabilities in MS patients. This case study highlights the recent work of Dr. Paul Tesar and colleagues at the Case Western Reserve University School of Medicine, which could potentially lead to such novel treatments, as it aims to control the function of stem cells in the body and thereby to help the body repair itself.

产品与服务 (9)
Filters:

1-9 的 9 产品与服务

  • 1000x1000_Operetta

    Operetta CLS 高内涵系统

    Operetta CLS? 系统帮助您探索常规实验与复杂创新实验中的生物学信息。通过一系列创?#24405;?#26415;的有机结合,Operetta CLS? 同时满足精细细胞细节成像对高速、高灵敏?#21462;?#39640;分辨率检测多方面的需求。同时,凭借功能强大的Harmony 4.5版软件,Operetta CLS? 可帮助您发现并定量细微的细胞表型改变。
  • EnSight Multimode Plate Reader

    EnSight 多模式读板仪

    EnSight? 多模式读板仪是一台整合了多标记、无标记和显微成像技术的桌面式一体化检测平台。其整孔成像&在线数据分析速?#30830;?#24120;快,可以在五分钟内完成384?#35013;?#25972;板的图像获取及分析。该仪器采用预置模块化设计,结合基于Kaleido软件的数据采集和分析流程,是一台真正的全能且高产的多模式读板仪。
  • 1000x1000_Opera_Phenix

    Opera Phenix 高内涵系统

    Opera Phenix?高内涵系统是目?#30333;?#21463;期待的高内涵共聚焦技术方案。?#35272;到?#36817;20年的顶尖高内涵产品opera高内涵成像系统积累的丰富成像和应用经验,新一代的opera phenix系统系统专为高内涵表型筛选以?#26696;?#26434;的疾病模型量身打造,例如活细胞、原代细胞,细胞微组织。
  • ATPlite 1-step 3D kit

    ATPlite 1Step 3D, 10 mL kit

    ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids. The 1-step format only requires one addition step, and can be used for continuous processing.
  • ATPlite 3D Kit

    ATPlite 3D, 300 assay point kit

    ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids.
  • CellCarrier Spheroid ULA 96-well Microplates

    Round bottom, clear 96-well polystyrene microplates coated with Ultra Low Attachment (ULA) surface for 3D culture of mammalian cells. This ULA coating in round-bottom wells facilitates formation of round spheroids after incubating seeded cells.
  • Columbus Image Data Storage and Analysis System

    Columbus Image Data Storage and Analysis System

    Our Columbus? Image Data Storage and Analysis system is an instrument agnostic image analysis and management platform. The Columbus system is the only system that provides universal high-volume image data storage and analysis and brings access to images from a wide range of sources including all major high content screening instruments.
  • Harmony Software 1

    Harmony High-Content Imaging and Analysis Software

    Easily quantify complex cellular phenotypes with Harmony high-content analysis software. Harmony software is designed for Operetta CLS? and Opera Phenix? high-content screening systems.

    Coming soon!
    Our forthcoming release includes an updated version of PreciScan, which significantly reduces imaging times and data volumes for 3D cell models grown in hydrogels.

  • PreciScan intelligent acquisition software lets you first pre-scan at low magnification

    PreciScan Intelligent Acquisition

    Harness the power of intelligent image acquisition with PreciScan for more efficient high-content imaging and analysis. This optional plug-in for Harmony high-content analysis software enables you to more accurately target your object of interest for significantly reduced acquisition and analysis times, particularly valuable for 3D microtissue and rare event studies.
香港黄大仙码报要钱料 河北福彩快三开奖公告 黑龙江体彩6+1走势图 熊猫麻将官方版下载 川崎前锋贵州人和 时时彩最长历史记录 天津11选5app下载 安徽时时彩开奖直播 8闽福建麻将 韦斯卡球场 亚冠塔什干棉农对吉达国民